Keratin Acidic (AE1) 抗体 (Biotin)
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北京 101111
Quick Overview for Keratin Acidic (AE1) 抗体 (Biotin) (ABIN6178735)
抗原
See all Keratin Acidic (AE1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Cytokeratin, Acidic (KRTL/1377), Biotin Conjugate
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产品特性
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human KRT77 protein fragment (exact sequence is proprietary)
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亚型
- IgG1
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anti-Keratin Acidic (AE1) antibody
AE1 适用: 人 IHC, WB 宿主: 大鼠 Monoclonal OTI1D10 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鸡, Cow, 小鼠, 兔, 猴, Pig IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鸡, Cow, 小鼠, 兔, 猴, 犬, Turtle IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IF, IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IF, IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
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应用备注
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Immunohistology (formalin) 1-2 μg/mL
- Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Skin, Squamous cell carcinoma (SCC)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Keratin Acidic (AE1)
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别名
- Cytokeratin, Acidic
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背景
- K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B
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分子量
- 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19)
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基因ID
- 374454, 334989
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UniProt
- Q7Z794
抗原
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