GJB1 抗体 (CF®405S)
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北京 101111
Quick Overview for GJB1 抗体 (CF®405S) (ABIN6178402)
抗原
See all GJB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Connexin-32 (GJB1/1753), CF405S Conjugate
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产品特性
- This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane ?-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human GJB1 protein
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亚型
- IgG
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anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (C-Term) antibody
GJB1 适用: 人, 大鼠, 小鼠, 犬, Cow, 马, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (Internal Region) antibodyGJB1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (AA 201-283) antibodyGJB1 适用: 人, 大鼠 ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (AA 215-283) antibodyGJB1 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) antibodyGJB1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB5895 unconjugated Recombinant Antibody
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (C-Term) antibodyGJB1 适用: 人, 大鼠, 小鼠, 犬, Cow, 猴, Pig, 马, 兔, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) antibodyGJB1 适用: 人, 大鼠, 小鼠 WB, FACS, IF, IHC (fro) 宿主: 小鼠 Monoclonal M12-13 unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) antibodyGJB1 适用: 人, 大鼠, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal GJB1-1753 unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (C-Term) antibodyGJB1 适用: 人, 大鼠, 小鼠, 犬, Cow, 猴, Pig, 马, 兔, 豚鼠, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, beta 1, 32kDa (GJB1) (C-Term) antibodyGJB1 适用: 人, 大鼠, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin) 1-2 μg/mL
- Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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MCF-7 Cells. Liver, Kidney, Stomach or Tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
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别名
- Connexin-32
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背景
- Charcot Marie Tooth neuropathy X linked, CMTX, CMTX1, Connexin-32, Cx32, GAP junction 28 kDa liver protein, Gap junction beta-1 protein, Gap junction protein beta 1 32kD, GJB1
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分子量
- 27-32 kDa
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基因ID
- 2705, 333303
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UniProt
- P08034
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途径
- Cell-Cell Junction Organization
抗原
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