BRCA1 抗体 (AA 445-620) (CF®647)
Quick Overview for BRCA1 抗体 (AA 445-620) (CF®647) (ABIN6175484)
抗原
See all BRCA1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 445-620
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原理
- Mouse Monoclonal anti-BRCA1 (BRCA1/1398), CF647 Conjugate
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产品特性
- The BRCA1 gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)
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亚型
- IgG1
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anti-Breast Cancer 1 (BRCA1) (AA 19-83) antibody
BRCA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (pSer1423) antibodyBRCA1 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 229-335) antibodyBRCA1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 6C6D2 unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 1661-1863) antibodyBRCA1 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (Ser184), (Ser186) antibodyBRCA1 适用: 人, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 445-620) antibodyBRCA1 适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal BRCA1-1398 unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 1314-1600), (AA 1314-1864) antibodyBRCA1 适用: 人 WB, IF, IP, FACS, ICC 宿主: 小鼠 Monoclonal RAY unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 64-160) antibodyBRCA1 适用: 大鼠, 小鼠 ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (pSer1524) antibodyBRCA1 适用: 人 ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 1524-1781) antibodyBRCA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- BRCA1 (Breast Cancer 1 (BRCA1))
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别名
- BRCA1
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分子量
- 220 kDa
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基因ID
- 672, 194143
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UniProt
- P38398
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途径
- Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
抗原
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