TGFA 抗体 (AA 34-43) (CF®405S)
Quick Overview for TGFA 抗体 (AA 34-43) (CF®405S) (ABIN6171410)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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                                            抗原表位
- AA 34-43
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                                            原理
- Mouse Monoclonal anti-TGF alpha (P/T1), CF405S Conjugate
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                                            产品特性
- This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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                                            免疫原
- A 10-amino acid synthetic peptide (aa34-43, PPVAAAVVSH) from human TGF?.
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                                            亚型
- IgG1 kappa
 
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                                            应用备注
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                        Immunohistology formalin-paraffin 2-4 μg/mL- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
 
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                                            说明
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                        Jurkat or Ramos cells. Testicular Carcinoma 
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                                            限制
- 仅限研究用
 
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                                            状态
- Liquid
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                                            浓度
- 100 μg/mL
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                                            缓冲液
- PBS/0.1 % BSA/0.05 % azide
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                                            储存液
- Sodium azide
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                                            注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            注意事项
- Protect from light
 
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    - TGFA (Transforming Growth Factor, alpha (TGFA))
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                                            别名
- TGF alpha
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                                            背景
- EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1
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                                            分子量
- 6 kDa
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                                            基因ID
- 7039, 170009
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                                            UniProt
- P01135
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                                            途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
 抗原
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