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TGFA 抗体 (C-Term) (CF®488A)

This anti-TGFA antibody is a 小鼠 单克隆 antibody detecting TGFA in IF, FACS 和 IHC (f). Suitable for 人, 大鼠, 小鼠 和 Cow.
产品编号 ABIN6171394
发货至: 中国

Quick Overview for TGFA 抗体 (C-Term) (CF®488A) (ABIN6171394)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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人, 大鼠, 小鼠, Cow

宿主

  • 77
  • 57
  • 4
  • 3
  • 2
小鼠

克隆类型

  • 75
  • 67
  • 1
单克隆

标记

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  • 11
  • 4
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  • 3
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This TGFA antibody is conjugated to CF®488A

应用范围

  • 79
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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

MF9
  • 抗原表位

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    • 11
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    C-Term

    原理

    Mouse Monoclonal anti-TGF alpha (MF9), CF488A Conjugate

    产品特性

    This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF?.

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 2-4 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Jurkat or Ramos cells. Testicular Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGF alpha

    背景

    EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

    分子量

    6 kDa

    基因ID

    7039, 170009

    UniProt

    P01135

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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