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HSPD1 抗体 (CF®488A)

This anti-HSPD1 antibody is a 小鼠 单克隆 antibody detecting HSPD1 in WB, IF, FACS, IHC (f) 和 ISt. Suitable for 人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig 和 绵羊.
产品编号 ABIN6164267
发货至: 中国

Quick Overview for HSPD1 抗体 (CF®488A) (ABIN6164267)

抗原

See all HSPD1 抗体
HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

适用

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人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig, 绵羊

宿主

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小鼠

克隆类型

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单克隆

标记

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This HSPD1 antibody is conjugated to CF®488A

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

克隆位点

HSPD1-780
  • 原理

    Mouse Monoclonal anti-HSP60 (HSPD1/780), CF488A Conjugate

    产品特性

    This antibody recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp?s), more appropriately called as stress response proteins (srp?s). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human HSPD1 protein

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.25-0.5 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

    别名

    HSP60

    分子量

    60 kDa

    基因ID

    3329, 595053

    UniProt

    P10809

    途径

    Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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