Calnexin 抗体 (N-Term)
Quick Overview for Calnexin 抗体 (N-Term) (ABIN6161468)
抗原
See all Calnexin (CANX) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Mouse Monoclonal anti-Calnexin (CANX/1541), Purified, with BSA
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产品特性
- This antibody recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)
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亚型
- IgG2b kappa
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anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, Pig, 猴, Hamster, 绵羊, 小鸡, Avian, 马, 山羊, Cat, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Calnexin (CANX) (Internal Region) antibodyCANX 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 犬 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 263-482) antibodyCANX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 504-592) antibodyCANX 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1541 unconjugated
anti-Calnexin (CANX) (AA 239-461) antibodyCANX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 239-461) antibodyCANX 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1543 unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 犬 WB, IHC, IF, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- PBS/0.05 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Calnexin (CANX)
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别名
- Calnexin
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背景
- Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90
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分子量
- 90 kDa
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基因ID
- 821, 567968
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UniProt
- P27824
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途径
- MAPK Pathway, Thyroid Hormone Synthesis
抗原
-
