Topoisomerase II alpha 抗体 (AA 1352-1493) (Biotin)
Quick Overview for Topoisomerase II alpha 抗体 (AA 1352-1493) (Biotin) (ABIN6157548)
抗原
See all Topoisomerase II alpha (TOP2A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1352-1493
-
原理
- Mouse Monoclonal anti-Topoisomerase-II-alpha (TOP2A/1361), Biotin Conjugate
-
产品特性
- This antibody recognizes a 170 kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with topoisomerase IIβ or topoisomerase I. Topo IIα plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIα is also implicated in drug resistance of tumor cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)
-
亚型
- IgG2b kappa
-
-
-
-
应用备注
-
Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
HeLa Cells or Tonsil
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
-
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
-
别名
- Topoisomerase-II-alpha
-
分子量
- 170 kDa
-
基因ID
- 7153, 156346
-
UniProt
- P11388
-
途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
-