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Recombinant Melanoma gp100 抗体 (CF®640R)

This anti-Melanoma gp100 antibody is a 兔 单克隆 antibody detecting Melanoma gp100 in IHC, IF 和 FACS. Suitable for 人.
产品编号 ABIN6154749
发货至: 中国

Quick Overview for Recombinant Melanoma gp100 抗体 (CF®640R) (ABIN6154749)

抗原

See all Melanoma gp100 (PMEL) 抗体
Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Melanoma gp100 antibody is conjugated to CF®640R

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

PMEL-1825R
  • 原理

    Recombinant Rabbit Monoclonal anti-gp100 / Melanosome / PMEL17 / SILV (PMEL/1825R), CF640R Conjugate

    产品特性

    Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human SILV protein

    亚型

    IgG kappa
  • 应用备注

    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    SK-MEL-28 cells or Melanoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

    别名

    gp100 / Melanosome / PMEL17 / SILV

    分子量

    90-100 kDa

    基因ID

    6490, 95972

    UniProt

    P40967
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