CD59 抗体
Quick Overview for CD59 抗体 (ABIN6151780)
抗原
See all CD59 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-CD59 (heavy chain of Protectin) (MACIF/629), Purified, BSA-free
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产品特性
- This antibody reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Recombinant full-length human CD59 protein
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亚型
- IgG1 kappa
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anti-CD59 (CD59) (AA 31-111) antibody
CD59 适用: 人 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8D2B8 unconjugated
anti-CD59 (CD59) (AA 74-110) antibodyCD59 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB56691 unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人, 小鼠 WB, FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-43-5 unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人 FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-43 unconjugated
anti-CD59 (CD59) (AA 31-111) antibodyCD59 适用: 人 WB, FACS, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-CD59 (CD59) (AA 23-105) antibodyCD59 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) (AA 1-128) antibodyCD59 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G6 unconjugated
anti-CD59 (CD59) (AA 52-100) antibodyCD59 适用: 人 FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) (AA 26-102) antibodyCD59 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2E11B6 unconjugated
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应用备注
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Tonsil, Cervix or Bladder.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS (no BSA, no azide)
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储存液
- Azide free
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- CD59
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别名
- CD59 (heavy chain of Protectin)
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分子量
- 20 kDa
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基因ID
- 966, 278573
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UniProt
- P13987
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途径
- Complement System
抗原
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