MCAM 抗体 (Biotin)
-
- 抗原 See all MCAM 抗体
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This MCAM antibody is conjugated to Biotin
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-CD146 / MCAM / MUC18 / Mucin 18 (C146/634), Biotin Conjugate
- 产品特性
- The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant human CD146 protein
- 克隆位点
- C146-634
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MCAM Primary Antibody
-
-
- 应用备注
-
Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
-
A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
- 抗原
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- 别名
- CD146 / MCAM / MUC18 / Mucin 18 (MCAM 产品)
- 别名
- CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
- 分子量
- 130 kDa
- 基因ID
- 4162, 599039
- UniProt
- P43121
-