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ADAM10 抗体 (APC)

ADAM10 适用: 人 FACS 宿主: 小鼠 Monoclonal 163003 APC
产品编号 ABIN602624
发货至: 中国
  • 抗原 See all ADAM10 抗体
    ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This ADAM10 antibody is conjugated to APC
    应用范围
    • 43
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    Flow Cytometry (FACS)
    特异性
    Recognizes human ADAM10 (APC).
    纯化方法
    Purified
    免疫原
    Mouse myeloma cell line NS0-derived recombinant human ADAM10 (aa Thr214 - Glu672, UniProt Accession #O14672).
    克隆位点
    163003
    亚型
    IgG2b
    Top Product
    Discover our top product ADAM10 Primary Antibody
  • 应用备注
    Approved: Flo

    Usage: Suitable for use in Flow Cytometry Flow Cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4 % paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1 % saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). 10 μL of undiluted product can be used to stain 1 x 10e6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    40-50 % glycerol, PBS.
    浓度
    Lot specific
    缓冲液
    Lyophilized from 25 μg antibody, 1 mL PBS, 0.1 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing
    储存条件
    4 °C,-20 °C
    储存方法
    May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C.
  • 抗原
    ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
    别名
    ADAM10 (ADAM10 产品)
    背景
    Name/Gene ID: ADAM10
    Subfamily: Metallopeptidase M12B
    Family: Protease

    Synonyms: ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM
    基因ID
    102
    UniProt
    O14672
    途径
    Notch Signaling, EGFR Signaling Pathway
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