ADAM10 抗体 (APC)
Quick Overview for ADAM10 抗体 (APC) (ABIN602624)
抗原
See all ADAM10 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes human ADAM10 (APC).
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纯化方法
- Purified
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免疫原
- Mouse myeloma cell line NS0-derived recombinant human ADAM10 (aa Thr214 - Glu672, UniProt Accession #O14672).
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亚型
- IgG2b
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anti-ADAM Metallopeptidase Domain 10 (ADAM10) (AA 420-641) antibody
ADAM10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) antibodyADAM10 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6C3C7 unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) (AA 400-414), (Extracellular) antibodyADAM10 适用: 小鼠 WB, IHC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) (AA 501-600) antibodyADAM10 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) antibodyADAM10 适用: 人 WB, FACS, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 11G2 unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) (AA 732-748) antibodyADAM10 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) antibody (PE)ADAM10 适用: 人 FACS 宿主: 小鼠 Monoclonal 11G2 PE
anti-ADAM Metallopeptidase Domain 10 (ADAM10) (AA 214-500) antibodyADAM10 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) antibodyADAM10 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 10 (ADAM10) (AA 218-445) antibodyADAM10 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: Flo
Usage: Suitable for use in Flow Cytometry Flow Cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4 % paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1 % saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). 10 μL of undiluted product can be used to stain 1 x 10e6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 40-50 % glycerol, PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from 25 μg antibody, 1 mL PBS, 0.1 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C.
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- ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
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别名
- ADAM10
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背景
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Name/Gene ID: ADAM10
Subfamily: Metallopeptidase M12B
Family: Protease
Synonyms: ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM -
基因ID
- 102
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UniProt
- O14672
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途径
- Notch Signaling, EGFR Signaling Pathway
抗原
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