IL12 抗体
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北京 101111
Quick Overview for IL12 抗体 (ABIN596134)
抗原
See all IL12 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes both the p35 and p40 sub-units of canine IL-12. Cross-reactivity (direct ELISA): ~40 % with recombinant human IL-12 p35/p40 and recombinant feline IL-12 p35/p40, <10 % with recombinant rat IL-12 p35/p40.
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纯化方法
- Affinity purified
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过滤
- 0.2 μm filtered
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免疫原
- CHO-derived recombinant canine interleukin 12 p40 (aa23-329, Q28268) and p35 (aa 26-222, Q28267).
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亚型
- IgG
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anti-Interleukin 12 (IL12) (AA 51-150) antibody
IL12 适用: 人 ELISA, WB, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 (IL12) (AA 168-195) antibodyIL12 适用: 人 ELISA, WB, FACS, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 (IL12) antibodyIL12 适用: 人 ELISA, WB, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 12 (IL12) antibodyIL12 适用: 小鼠 ELISA, WB, Neut 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Approved: ELISA, Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Neutralization. ELISA (capture): 0.2-0.8 μg/mL. Western Blot: 0.1-0.2 μg/mL. Detection limit for rcaIL-12 is ~50 ng/lane under non-reducing and reducing conditions. Neutralization: The ND 50 was determined to be ~0.1-0.5 μg/mL in the presence of 25 ng/mL of rcaIL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs). -
说明
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Target Species of Antibody: Dog
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS, 40-50 % glycerol
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浓度
- Lot specific
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缓冲液
- Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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- IL12 (Interleukin 12 (IL12))
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别名
- IL12
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背景
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Name/Gene ID: IL-12
Synonyms: IL-12 -
途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原
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