NRF2 抗体 (C-Term)
Quick Overview for NRF2 抗体 (C-Term) (ABIN5706863)
抗原
See all NRF2 (NFE2L2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-gt6
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Supplier
- Rockland
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原理
- Nrf2 Antibody
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交叉反应 (详细)
- This affinity-purified antibody is directed against mouse Nrf2 protein.
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产品特性
- Synonyms: rabbit anti-Nrf2 antibody, Nrf 2, Nrf-2 Antibody, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, Nfe2l2, HEBP1
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: This affinity purified Nrf2 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66.9 kDa in size corresponding to Nrf-2 proteins by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1.0 μg/mL
ELISA Dilution: 5 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
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别名
- Nfe2l2
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背景
- Background: Anti-Nrf-2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nrf2 links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Nrf2 is localized in the cytoplasm and nucleus and ubiquitously expressed with the highest concentrations in the kidney, muscle, lung, heart, liver, and brain. Anti-Nrf2 antibody is useful for researchers interested in cellular growth, transcription factor, and cancer research.
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基因ID
- 18024
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NCBI登录号
- NP_035032
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UniProt
- Q60795
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途径
- ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
抗原
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