NEDD4-2 抗体 (AA 703-975)
Quick Overview for NEDD4-2 抗体 (AA 703-975) (ABIN5693274)
抗原
See all NEDD4-2 (NEDD4L) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 703-975
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原理
- Anti-NEDD4-2/NEDD4L Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NEDD4-2/NEDD4L Antibody Picoband® (ABIN5693274). Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human NEDD4-2 recombinant protein (Position: N703-D975).
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亚型
- IgG
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anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 1-100) antibody
NEDD4L 适用: 人 ELISA, WB, IP 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 836-955) antibodyNEDD4L 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) antibodyNEDD4L 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 289-494) antibodyNEDD4L 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) antibodyNEDD4L 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (pSer448) antibodyNEDD4L 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (Middle Region) antibodyNEDD4L 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 1-100) antibodyNEDD4L 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 275-325) antibodyNEDD4L 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig WB, IHC, IF, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 225-275) antibodyNEDD4L 适用: 人, 小鼠, Cow, Pig, 兔 WB, IHC, IF, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Broix, L., Jagline, H., Ivanova, E. L., Schmucker, S., Drouot, N., Clayton-Smith, J., Pagnamenta, A. T., Metcalfe, K. A., Isidor, B., Louvier, U. W., Poduri, A., Taylor, J. C., and 28 others.Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia. Nature Genet. 48: 1349-1358, 2016. 2. Dunn, D. M., Ishigami, T., Pankow, J., von Niederhausern, A., Alder, J., Hunt, S. C., Leppert, M. F., Lalouel, J.-M., Weiss, R. B. Common variant of human NEDD4L activates a cryptic splice site to form a frameshifted transcript. J. Hum. Genet. 47: 665-676, 2002. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))
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别名
- NEDD4L
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背景
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Synonyms: E3 ubiquitin-protein ligase NEDD4-like, HECT-type E3 ubiquitin transferase NED4L, NEDD4.2, Nedd4-2, NEDD4L, KIAA0439, NEDL3
Tissue Specificity: Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).
Background: Neural precursor cell expressed developmentally downregulated gene 4-like (NEDD4L) or NEDD4-2 (NEDD4-2) is an enzyme (ubiquitin ligase) of the NEDD4 family. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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分子量
- 130 kDa
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基因ID
- 23327
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途径
- Negative Regulation of Transporter Activity
抗原
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