PERK 抗体 (AA 222-334)
(1 reference)Quick Overview for PERK 抗体 (AA 222-334) (ABIN5693216)
抗原
See all PERK (EIF2AK3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 222-334
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原理
- Anti-PERK/EIF2AK3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PERK/EIF2AK3 Antibody Picoband® (ABIN5693216). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human PERK recombinant protein (Position: R222-Q334).
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 973-1114) antibody
EIF2AK3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr982) antibodyEIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr980) antibodyEIF2AK3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (C-Term) antibodyEIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 1001-1116) antibodyEIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 115-330) antibodyEIF2AK3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 665-764) antibodyEIF2AK3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (N-Term) antibodyEIF2AK3 适用: 人 WB, IHC, IF, IP, IC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr981) antibodyEIF2AK3 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 665-764) antibodyEIF2AK3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7B10 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Harding, H. P., Zhang, Y., Bertolotti, A., Zeng, H., Ron, D. Perk is essential for translational regulation and cell survival during the unfolded protein response. Molec. Cell 5: 897-904, 2000. 2. Hayes, S. E., Conner, L. J., Stramm, L. E., Shi, Y. Assignment of pancreatic eIF-2a kinase (EIF2AK3) to human chromosome band 2p12 by radiation hybrid mapping and in situ hybridization. Cytogenet. Cell Genet. 86: 327-328, 1999. 3. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." in: American journal of cancer research, Vol. 6, Issue 5, pp. 924-36, (2016) (PubMed).
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: "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." in: American journal of cancer research, Vol. 6, Issue 5, pp. 924-36, (2016) (PubMed).
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- PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
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别名
- EIF2AK3
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背景
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Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK
Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.
Background: Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
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分子量
- 140 kDa
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基因ID
- 9451
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途径
- Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response
抗原
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