14-3-3 sigma/SFN 抗体 (AA 1-248)
(1 reference)Quick Overview for 14-3-3 sigma/SFN 抗体 (AA 1-248) (ABIN5693156)
抗原
See all 14-3-3 sigma/SFN (SFN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-248
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原理
- Anti-14-3-3 sigma/SFN Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-14-3-3 sigma/SFN Antibody Picoband® (ABIN5693156). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).
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亚型
- IgG
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anti-Stratifin (SFN) antibody
SFN 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (Internal Region) antibodyVerified SFN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Stratifin (SFN) (C-Term) antibodySFN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 140-156) antibodySFN 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-249) antibodySFN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Stratifin (SFN) antibodySFN 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-SFN-2 unconjugated
anti-Stratifin (SFN) (AA 140-156), (Middle Region) antibodySFN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993. 2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002. 3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Makorin-2 is a neurogenesis inhibitor downstream of phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8486-95, (2008) (PubMed).
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: "Makorin-2 is a neurogenesis inhibitor downstream of phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8486-95, (2008) (PubMed).
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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别名
- SFN
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背景
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Synonyms: 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1
Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Background: Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
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分子量
- 28 kDa
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基因ID
- 2810
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UniProt
- P31947
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途径
- p53 Pathway, Myometrial Relaxation and Contraction
抗原
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