MYLK 抗体 (AA 1441-1709)
Quick Overview for MYLK 抗体 (AA 1441-1709) (ABIN5693029)
抗原
See all MYLK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1441-1709
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原理
- Anti-Myosin light chain kinase/MYLK Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Myosin light chain kinase/MYLK Antibody Picoband® (ABIN5693029). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human MYLK recombinant protein (Position: D1441-D1709).
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亚型
- IgG
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anti-Myosin Light Chain Kinase (MYLK) (AA 1375-1524) antibody
MYLK 适用: 人, 小鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7F2B11 unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1684-1884) antibodyMYLK 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1765-1914) antibodyMYLK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 923-953), (N-Term) antibodyMYLK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB03067 unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 908-938) antibodyMYLK 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1661-1877) antibodyMYLK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1-992) antibodyMYLK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (C-Term) antibodyMYLK 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 290-398) antibodyMYLK 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1-992) antibodyMYLK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: MYLK myosin, light chain kinase". 2. Connell LE, Helfman DM (2007). "Myosin light chain kinase plays a role in the regulation of epithelial cell survival". J. Cell. Sci. 119 (Pt 11): 2269-81. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MYLK (Myosin Light Chain Kinase (MYLK))
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别名
- MYLK
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背景
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Synonyms: Myosin light chain kinase, smooth muscle, MLCK, s mMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1
Tissue Specificity: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
Background: Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin.
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分子量
- 250 kDa
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基因ID
- 4638
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UniProt
- Q15746
抗原
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