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CYBB 抗体 (AA 416-500)

Name: CYBB 抗体 (AA 416-500)
SKU: ABIN5692996
Availability: OutOfStock

CYBB 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot analysis of NOX2 using anti-NOX2 antibody . (CYBB 抗体 (AA 416-500))

1 image

(4 references)

产品编号 ABIN5692996

发货至: 中国

Datasheet as PDF

抗原 See all CYBB 抗体

CYBB (Cytochrome B-245, beta Polypeptide (CYBB))
抗原表位
15

6

4

3

2

1

1

1

1

1

1

1

1

1
AA 416-500
适用
55

37

36

19

15

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
51

4

2
兔
Compatible Secondaries
克隆类型
43

14
多克隆
标记
24

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CYBB antibody is un-conjugated
应用范围
35

18

16

12

12

8

6

6

3

3

3
Western Blotting (WB), ELISA

原理

Anti-NOX2/gp91phox/CYBB Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins.

产品特性

Anti-NOX2/gp91phox/CYBB Antibody Picoband® (ABIN5692996). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

免疫原

E. coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).

亚型

IgG

Top Product

Discover our top product CYBB Primary Antibody
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (C-Term) antibody
CYBB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) antibody
CYBB 适用: 人 WB, IF, IHC (p), ICC 宿主: 小鼠 Monoclonal 54-1 unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 442-459), (Middle Region) antibody
CYBB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (Internal Region) antibody
Verified CYBB 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 100-200) antibody
CYBB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody (Biotin)
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody (AbBy Fluor® 350)
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 350

应用备注

Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bionda, C., Li, X. J., van Bruggen, R., Eppink, M., Roos, D., Morel, F., Stasia, M.-J. Functional analysis of two-amino acid substitutions in gp91phox in a patient with X-linked flavocytochrome b(558)-positive chronic granulomatous disease by means of transgenic PLB-985 cells. Hum. Genet. 115: 418-427, 2004. 2. Bolscher, B. G. J. M., de Boer, M., de Klein, A., Weening, R. S., Roos, D. Point mutations in the beta-subunit of cytochrome b(558) leading to X-linked chronic granulomatous disease. Blood 77: 2482-2487, 1991. 3. "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)".

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Zhang, Deng, Lai, Guan, Sun, Han, Wang, Pan, Ji, Luo, Huang, Tang, Gu, Dan, Yu, Namaka, Zhang, Deng, Li: "Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation." in: Scientific reports, Vol. 6, pp. 30146, (2018) (PubMed).

Deep, Kumar, Jain, Dhar, Panigrahi, Hussain, Agarwal, El-Elimat, Sica, Oberlies, Agarwal: "Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity." in: Scientific reports, Vol. 6, pp. 23135, (2017) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." in: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Du, Yang, Zhou, Liu, Li, Chen, Gao: "D?galactose?induced mitochondrial DNA oxidative damage in the auditory cortex of rats." in: Molecular medicine reports, Vol. 10, Issue 6, pp. 2861-7, (2014) (PubMed).
抗原

CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

别名

CYBB (CYBB 产品)

背景

Synonyms: Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b (558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, CYBB, NOX2
Tissue Specificity: Detected in neutrophils (at protein level).
Background: NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

分子量

65 kDa

基因ID

1536

UniProt

P04839