FASL 抗体 (AA 196-220)
Quick Overview for FASL 抗体 (AA 196-220) (ABIN5690653)
抗原
See all FASL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 196-220
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纯化方法
- FasL (monoclonal A11) antibody was purified to ≥,95% in SDS-PAGE.
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免疫原
- FasL (monoclonal A11) antibody was raised against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
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亚型
- IgM
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anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 196-281) antibody
FASL 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-200) antibodyFASL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 102-280) antibodyFASL 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-278) antibodyFASL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 103-281) antibodyFASL 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 82-145) antibodyFASL 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) antibodyFASL 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2G9-G8 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal NOK-1 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal NOK-1 unconjugated
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应用备注
- FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunohistochemistry (frozen sections and paraffin sections).Method: Cryostat sections (6-8 μ,m) were air dried, fixed in 4 % paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 μ,g/mL of A11 antibody in PBS, 0.2 % BSA, 0.05 % Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.Paraffin sections: Dewax in xylene (2x10 min), EtOH 100 % (2x10 min), block endogeneous peroxidase (0.6 % H2O2 in Methanol 10 min), rehydrate through graded EtOH (100 %, 95 %, 70 %), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 μ,g/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS containing 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- FasL antibody can be stored at 4°C, stable for one year.
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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别名
- FasL
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基因ID
- 14103
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UniProt
- P41047
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途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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