EGF 抗体
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北京 101111
Quick Overview for EGF 抗体 (ABIN5690467)
抗原
See all EGF 抗体适用
宿主
克隆类型
标记
应用范围
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Rat EGF specific antibody was purified by affinity chromatography employing immobilized Rat EGF matrix.
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anti-Epidermal Growth Factor (EGF) (AA 690-720), (Center) antibody
EGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB16555 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibodyEGF 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 9D7F11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1000-1060) antibodyEGF 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibodyEGF 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 609-751) antibodyEGF 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 小鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 31-53) antibodyEGF 适用: 人 IF (p), IF (cc), IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of < 0.1 μ,g/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat EGF by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Sandwich
To detect Rat EGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Western Blot:
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- AntiEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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背景
- Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor is also a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways
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基因ID
- 25313
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UniProt
- P07522
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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