CDC25A 抗体 (pSer76)
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Quick Overview for CDC25A 抗体 (pSer76) (ABIN5689805)
抗原
See all CDC25A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer76
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特异性
- This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
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纯化方法
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
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免疫原
- cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.
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应用备注
- Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store antibody at -20°C for up to one year.
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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别名
- cdc25A
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背景
- Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
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分子量
- 70 kDa
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基因ID
- 993
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NCBI登录号
- NP_001780
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UniProt
- P30304
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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