EGF 抗体
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北京 101111
Quick Overview for EGF 抗体 (ABIN5688983)
抗原
See all EGF 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hEGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF.
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anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 690-720), (Center) antibodyEGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB16555 unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 9D7F11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1000-1060) antibodyEGF 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibodyEGF 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 609-751) antibodyEGF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 小鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1-30) antibodyEGF 适用: 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 μ,g/mL of this antibody is required.
ELISA:
To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich: To detect hEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.
Western Blot:
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- EGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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分子量
- 6.2 kDa
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基因ID
- 1950
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UniProt
- P01133
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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