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ERK1/2 抗体 (pThr202, pTyr204)

This anti-ERK1/2 antibody is a 兔 多克隆 antibody detecting ERK1/2 in WB 和 IHC. Suitable for 人, 小鼠, 大鼠 和 非洲爪蟾.
产品编号 ABIN5688926
发货至: 中国

Quick Overview for ERK1/2 抗体 (pThr202, pTyr204) (ABIN5688926)

抗原

See all ERK1/2 (MAPK1/3) 抗体
ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

适用

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人, 小鼠, 大鼠, 非洲爪蟾

宿主

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克隆类型

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多克隆

标记

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This ERK1/2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    pThr202, pTyr204

    特异性

    ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204..

    纯化方法

    Affinity Purified

    免疫原

    ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.
  • 应用备注

    Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

    储存条件

    -20 °C

    储存方法

    For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
  • 抗原

    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

    别名

    ERK1 / 2

    背景

    Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.

    分子量

    42/44 kDa

    基因ID

    116590

    UniProt

    P63086
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