SYN1 抗体 (pSer609)
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Quick Overview for SYN1 抗体 (pSer609) (ABIN5688923)
抗原
See all SYN1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer609
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特异性
- Synapsin antibody is specific for Synapsin I phosphorylated at Ser603.
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纯化方法
- Affinity Purified
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免疫原
- Synapsin (Ser603) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser609 of Rat Synapsin I.
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应用备注
- Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
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储存条件
- -20 °C
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储存方法
- For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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- SYN1 (Synapsin I (SYN1))
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别名
- Synapsin
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背景
- Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.
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分子量
- 78 kDa
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基因ID
- 281510
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UniProt
- P17599
抗原
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