TAO2 antibody is specific for Mr ~120k TAO2 phosphorylated at Ser181.
纯化方法
Affinity Purified
免疫原
TAO2 (Ser181) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser181 of TAO2.
Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
限制
仅限研究用
状态
Liquid
缓冲液
100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
储存条件
-20 °C
储存方法
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
抗原
TAO Kinase 2 (TAOK2)
别名
TAO2
背景
TAO (thousand and one amino acid) protein kinases mediate signaling from carbachol to p38 MAPK and ternary complex transcription factors. This has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs. Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.