NGFB 抗体
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北京 101111
Quick Overview for NGFB 抗体 (ABIN5688734)
抗原
See all NGFB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
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免疫原
- beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.
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anti-Nerve Growth Factor beta (NGFB) (AA 151-220) antibody
NGFB 适用: 人, 大鼠, 小鼠, 犬 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor beta (NGFB) (AA 84-115) antibodyNGFB 适用: 人 WB, IHC (p), FACS, IF 宿主: 兔 Polyclonal RB14403 unconjugated
anti-Nerve Growth Factor beta (NGFB) antibodyNGFB 适用: 人 WB, IHC, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 200 pg/mL of recombinant human β,-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect hβ,-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ,-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- 4 °C,-20 °C
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储存方法
- beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
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- NGFB (Nerve Growth Factor beta (NGFB))
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别名
- bNGF
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基因ID
- 4803
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UniProt
- P01138
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途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
抗原
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