CD40 Ligand 抗体 (Biotin)
Quick Overview for CD40 Ligand 抗体 (Biotin) (ABIN5687607)
抗原
See all CD40 Ligand (CD40LG) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hsCD40L specific antibody was purified by affinity chromatography and then biotinylated.
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免疫原
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (Human sCD40L).
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应用备注
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ELISA:
To detect hsCD40L by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsCD40L.
Sandwich:
To detect hsCD40L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L.
Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CD40 Ligand (CD40LG)
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别名
- sCD40 Ligand
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基因ID
- 959
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UniProt
- P29965
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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