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FASL 抗体 (Biotin)

FASL 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal Biotin
产品编号 ABIN5687581
发货至: 中国
  • 抗原 See all FASL 抗体
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    适用
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    宿主
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    山羊
    克隆类型
    • 73
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    多克隆
    标记
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    This FASL antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), ELISA
    纯化方法
    Anti-hFasL/Apo1 specific antibody was purified by affinity chromatography and then biotinylated.
    免疫原
    Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL/Apo1 (human FasL/Apo1).
    Top Product
    Discover our top product FASL Primary Antibody
  • 应用备注
    ELISA:

    To detect hFasL/Apo1 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFasL/Apo1.

    Sandwich: To detect hFasL/Apo1L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FasL/Apo1L (XP-5130) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFasL/Apo1L.

    Western Blot:

    To detect hFasL/Apo1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    Fas Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    别名
    Fas Ligand (FASL 产品)
    别名
    ALPS1B antibody, APT1LG1 antibody, APTL antibody, CD178 antibody, CD95-L antibody, CD95L antibody, FASL antibody, TNFSF6 antibody, fasl antibody, Fas-L antibody, Faslg antibody, Tnfsf6 antibody, gld antibody, Apt1Lg1 antibody, Fasl antibody, FASLG antibody, zgc:162027 antibody, Fas ligand antibody, Fas ligand L homeolog antibody, Fas ligand (TNF superfamily, member 6) antibody, FASLG antibody, faslg.L antibody, Fasl antibody, Faslg antibody, faslg antibody
    背景
    Fas Ligand is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
    基因ID
    356
    UniProt
    P48023
    途径
    Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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