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Recombinant His Tag 抗体

This anti-His Tag antibody is a Human Chimeric antibody detecting His Tag in WB, ELISA 和 FACS. Suitable for .

产品编号 ABIN5668258

发货至: 中国

Quick Overview for Recombinant His Tag 抗体 (ABIN5668258)

抗原

See all His Tag 抗体

His Tag

抗体类型

Recombinant Antibody

适用

请咨询

宿主

107
65
11
10
8
8
2
1

Human

克隆类型

Chimeric

标记

This His Tag antibody is un-conjugated

应用范围

167
115
40
32
31
30
28
16
15
13
13
13
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1

Western Blotting (WB), ELISA, Flow Cytometry (FACS)

克隆位点

3D5

Discover our top product His Tag Primary Antibody

原理

Anti-His tag (C-term) [3D5], Human IgG3, kappa

特异性

This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

产品特性

Original Species of Ab: Mouse
Original Format of Ab: IgG2b

纯化方法

Protein A affinity purified

免疫原

Purified his tagged Fab fragment.

亚型

IgG3 kappa
anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 800)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800

anti-His Tag antibody (DyLight 649)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

anti-His Tag antibody (HRP)
WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP

应用备注

This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997, PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015, PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019, PMID: 30885301).

说明

This is a chimeric human antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

PBS with 0.02 % Proclin 300.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
抗原

His Tag

物质类

Tag

背景

Polyhistidine tag

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