Recombinant EGFR 抗体 (Extracellular Domain)
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Quick Overview for Recombinant EGFR 抗体 (Extracellular Domain) (ABIN5668057)
抗原
See all EGFR 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Anti-EGFR [7A7], Rabbit IgG, kappa
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特异性
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Recognizes the extracellular domain of EGFR.
Application Notes original Clone: The antibody was generated in order to establish a mouse model for in vivo pre-clinical evaluation of therapies targeting EGFR. The antibody was able to recognize the murine and human EGFR expressed on 3LL or A431 tumor cell lines by flow cytometry. Moreover, the antibody could immunoprecipitate EGFR of 3LL cell lysate, it was used in immunohistochemistry on mouse skin tissue and mammary adenocarcinoma, and it was able to detect EGFR in Western blots (Garrido et al., 2004, PMID: 15312307). The antibody inhibited EGF-induced EGFR signaling in D122 tumor cells by reducing EGFR phosphorylation as well as the phosphorylation of molecules involved in downstream EGFR signaling, the antibody showed strong anti-metastatic activity on D122 tumors, for which the contribution of T cells is essential (Garrido et al., 2007). The crystal structure of the Fab fragment of the antibody was determined. The specificity of the antibody to extracellular EGFR was confirmed by ELISA, while competition ELISA showed the antibody does not compete with Nimotuzumab. The antibody recognized a binding site located on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment, such as with tyrosine kinase inhibitor (Talavera et al., 2011, PMID: 21592580). Talavera et al. observed the antibody recognition is strictly dependent on the experimental setup, and results can vary significantly, depending on seemingly minor experimental variations. In this regard, He et al. claimed to be unable to reproduce previous results obtained with the antibody. In their experiments the antibody did not detect EGFR expression by immunofluorescence or Western blot. Flow cytometry experiments showed surface expression of EGFR in A431 cells, weak staining in 3T3-L1 cells, and no staining on the surface of HPV38 cells. The antibody failed to detect EGFR on the plasma membrane of HPV38 tumour sections and in vivo administration of the antibody in an EGFR-expressing HPV38 tumour model did not have any impact on tumour regression or animal survival (He et al., 2018, PMID: 29552307). However, in 2021 Skibbe and coworker successfully used the antibody to treat mice with established colitis-driven CRCs. In particular, the antibody bound to EGFR by ELISA experiments, in vivo experiments showed the antibody significantly lowered the frequency of large tumors in mice and the antibody induced a significant downregulation of PD-L1 in highly glycolytic CRC cells (Skibbe et al., 2021, PMID: 34830971). Absolute antibody wanted to report these discrepancies in the results of different groups, suggesting that optimization of experimental conditions is crucial for the effectiveness of the antibody.
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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纯化方法
- Purified antibody.
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免疫原
- Recombinant extracellular domain of murine EGFR (rECD-mEGFR).
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亚型
- IgG, kappa
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5G9B5 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibodyEGFR 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5G9B5 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 小鸡, 犬, 马, 兔, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Mouse
OriginalFormat: IgG1
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限制
- 仅限研究用
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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别名
- EGFR
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背景
- ERBB, ERBB1, HER1, PIG61, mENA, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, epidermal growth f
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UniProt
- P00533
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
抗原
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