SYK 抗体 (pTyr525, pTyr526)
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Quick Overview for SYK 抗体 (pTyr525, pTyr526) (ABIN7676197)
抗原
See all SYK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr525, pTyr526
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交叉反应 (详细)
- Expected species reactivity: Mouse,Rat,Pig
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纯化方法
- Antigen affinity purified
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免疫原
- This phospho-Syk antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY525/526 of human Syk.
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亚型
- Ig Fraction
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应用备注
- Titration of the phospho-Syk antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the phospho-Syk antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- SYK (Spleen tyrosine Kinase (SYK))
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别名
- SYK
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背景
- Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.
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UniProt
- P43405
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
抗原
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