GFP 抗体
Quick Overview for GFP 抗体 (ABIN559689)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
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灵敏度
- WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
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纯化方法
- Protein A affinity chromatography from mouse ascites fluid
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免疫原
- GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
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亚型
- IgG2b
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应用备注
- WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
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限制
- 仅限研究用
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浓度
- 1mg/mL
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缓冲液
- 10mM PBS, pH 7.2, 0.05% NaN3
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).
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: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
抗原
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