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GFP 抗体

This anti-GFP antibody is a 小鼠 单克隆 antibody detecting GFP in WB, ELISA, IP, DB 和 ISt. Suitable for Aequorea victoria. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN559689
发货至: 中国

Quick Overview for GFP 抗体 (ABIN559689)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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小鼠

克隆类型

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单克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)

克隆位点

GF28R
  • 特异性

    Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

    灵敏度

    WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins

    纯化方法

    Protein A affinity chromatography from mouse ascites fluid

    免疫原

    GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

    亚型

    IgG2b
  • 应用备注

    WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

    限制

    仅限研究用
  • 浓度

    1mg/mL

    缓冲液

    10mM PBS, pH 7.2, 0.05% NaN3

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C
  • Chi, Bo, Wu, Jong, Huang: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP
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