alpha 2 Macroglobulin 抗体 (HRP)
Quick Overview for alpha 2 Macroglobulin 抗体 (HRP) (ABIN5596814)
抗原
See all alpha 2 Macroglobulin (A2M) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-103-207-0100
-
Supplier
- Rockland
-
原理
- Alpha-2-Macroglobulin Antibody Peroxidase Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma].
-
产品特性
- Synonyms: goat anti-Alpha-2-Macroglobulin Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody
-
纯化方法
- Anti-Alpha 2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
免疫原
-
Immunogen: a2-Macroglobulin [Human Plasma]
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-alpha-2-Macroglobulin (A2M) (AA 616-856) antibody
A2M 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 616-856) antibodyA2M 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1053-1214) antibodyA2M 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1216-1467) antibodyA2M 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1-1474) antibodyA2M 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 641-730) antibodyA2M 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, ELISA, IP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人, Pig, 兔, 犬 IHC, ELISA, IP, IEP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
-
Immunohistochemistry Dilution: 1:50 - 1:250
Application Note: Anti-Alpha 2-Macroglobulin has been tested by dot blot and western blot and is suitable to be assayed against 1.0 μg of alpha-Macroglobulin [Human Plasma] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:100 - 1:500
ELISA Dilution: 1:400 - 1:2,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 1 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
-
别名
- A2M
-
背景
- Background: Alpha 2-Macroglobulin is the largest major nonimmunoglobulin protein in plasma. The alpha 2-macroglobulin molecule is synthesized mainly in liver, but also locally by macrophages, fibroblasts, and adrenocortical cells. Alpha 2 macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. Alpha 2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β. Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
-
基因ID
- 2
-
NCBI登录号
- NP_000005
-
UniProt
- P01023
-
途径
- Lipid Metabolism
抗原
-
