CDKN1B 抗体 (pSer140)
(1 reference)
Quick Overview for CDKN1B 抗体 (pSer140) (ABIN5596806)
抗原
See all CDKN1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer140
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Supplier Product No.
- 600-401-k08
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Supplier
- Rockland
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原理
- p27 kip1 phospho S140 Antibody
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交叉反应 （详细）
- Anti-p27 kip1 pS140 is directed against the phosphorylated form of human p27 kip1 protein at the S140 residue.
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产品特性
- Synonyms: rabbit anti-p27 kip1 pS140 antibody, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Kip-1, KIP1, CDKN1B
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-P27 antibody
CDKN1B 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 150-198) antibodyCDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibodyCDKN1B 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4B4-E6 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyKO Validated CDKN1B 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SX53G8 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人, 小鼠, 大鼠, 猴 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal DCS-72-F6-KIP1-769 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 1G2 unconjugated Recombinant Antibody
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-p27kip1 pS140 antibody is tested for use in ELISA, immunoprecipitation, co-immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.
Western Blot Dilution: 1:500 - 1:4,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.68 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells." in: Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol. 1864, Issue 2, pp. 158-167, (2019) (PubMed).
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: "Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells." in: Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol. 1864, Issue 2, pp. 158-167, (2019) (PubMed).
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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别名
- CDKN1B
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背景
- Background: p27 kip1 pS140 is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Phosphorylation occurs on serine, threonine and tyrosine residues. Anti-p27 kip1 pS140 antibody is ideal for researchers interested in cell cycle and cancer research.
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基因ID
- 1027, 4757962
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UniProt
- P46527
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
抗原
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