PPARG 抗体 (pSer273)
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- 抗原 See all PPARG 抗体
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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抗原表位
- pSer273
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PPARG antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 600-401-j94
- Supplier
- Rockland
- 原理
- PPAR gamma phospho S273 Antibody
- 交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100 % sequence homology.
- 产品特性
- Synonyms: rabbit anti-PPAR gamma pS273 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma
- 纯化方法
- Anti-PPAR gamma pS273 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma at the S273 residue.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product PPARG Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-PPAR antibody has been tested by ELISA and dot blot and is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.85 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
- 别名
- PPARG (PPARG 产品)
- 背景
- Background: PPAR has a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. The receptor, when activated by ligand, binds to a promotor element in the gene for acyl-CoA oxidase and activates its transcription. In this way it controls the peroxisomal beta-oxidation pathway of fatty acids. It plays a key role in regulating adipocyte differentiation, gut homeostasis, and glucose homeostasis. Anti-PPAR gamma pS273 Antibody is ideal for researchers interested in Metabolism, Cardiovascular or Neuroscience research.
- 基因ID
- 5468, 20336229
- UniProt
- P37231
- 途径
- MAPK Pathway, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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