CD31 抗体 (C-Term)
Quick Overview for CD31 抗体 (C-Term) (ABIN5596763)
抗原
See all CD31 (PECAM1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
Supplier Product No.
- 100-401-gn4
-
Supplier
- Rockland
-
原理
- CD31 Antibody
-
交叉反应 (详细)
- CD31 PECAM1 antibody is directed against rat CD31 protein.
-
产品特性
- Synonyms: Rabbit anti-CD31 antibody, rabbit anti-PECAM-1 antibody, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD-31 Antibody, PECAM1 Antibody, CD31/PECAM1 Antibody
-
纯化方法
- The product is delipidated and defibrinated antiserum.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
-
-
应用备注
-
Immunohistochemistry Dilution: 4 μg/mL
Application Note: CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Western Blot Dilution: 1:500
ELISA Dilution: 1:20,000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 90 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
-
别名
- Pecam1
-
背景
- Background: Anti-CD31 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). CD31 (PECAM-1) is a 130 kDa platelet endothelial cell adhesion molecule encoded by the PECAM1 gene found on chromosome 17 in humans. CD31 is expressed on platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 plays a key role in modulation of integrin-mediated cell adhesion, leukocyte transendothelial migration, angiogenesis, apoptosis and macrophage phagocytosis. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. Anti-CD31 is ideal for researchers interested in Stem Cell Research, Epigenetics, and Cell-cycle Regulation research.
-
基因ID
- 29583
-
NCBI登录号
- NP_113779
-
UniProt
- Q3SWT0
-
途径
- Regulation of Actin Filament Polymerization
抗原
-