Fibronectin 1 抗体
Quick Overview for Fibronectin 1 抗体 (ABIN5596761)
抗原
See all Fibronectin 1 (FN1) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-117-0.5
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Supplier
- Rockland
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原理
- Fibronectin Antibody
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交叉反应 （详细）
- Typically less than 1 % cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
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产品特性
- Synonyms: rabbit anti-Fibronectin antibody, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3
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纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Fibronectin 1 (FN1) antibody
FN1 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Fibronectin 1 (FN1) antibody (Biotin)FN1 适用: 人, 小鼠, 大鼠, Cow, 猴 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
anti-Fibronectin 1 (FN1) (AA 1-163) antibodyFN1 适用: 人 WB, IF, PLA 宿主: 小鼠 Polyclonal unconjugated
anti-Fibronectin 1 (FN1) (C-Term) antibodyFN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin 1 (FN1) (AA 2200-2355) antibodyFN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin 1 (FN1) (C-Term) antibodyFN1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin 1 (FN1) antibodyFN1 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 9A5 unconjugated
anti-Fibronectin 1 (FN1) (C-Term) antibodyFN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin 1 (FN1) antibodyFN1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Fibronectin 1 (FN1) (AA 1268-1341) antibodyFN1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Rockland's anti-Fibronectin detects intact fibronectin (Invitrogen, Cat. No. 33016-015) by western blot after digestion by Matrix Metalloproteinase-3 (MMP-3) overnight at 37° C. Separation was performed using a 4-12 % Tris-Glycine gel. Under these conditions a sizeable, dark band at ~220 kDa representing the undigested fibronectin, as well as many, smaller bands representing the variably sized fragments resulting from fibronectin digestion by MMP-3 were noted. For immunohistochemistry paraffin embedded tissue preparation is recommended.
Western Blot Dilution: 1:5,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Fibronectin 1 (FN1)
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别名
- FN1
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背景
- Background: Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.
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基因ID
- 2335
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UniProt
- P02751
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途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
抗原
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