AKT 1/2/3 抗体
(1 reference)
Quick Overview for AKT 1/2/3 抗体 (ABIN5596659)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-n98
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Supplier
- Rockland
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原理
- AKT Antibody
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交叉反应 （详细）
- AKT Antibody reacts with the AKT from human tissues.
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产品特性
- Synonyms: rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, AKT3 Antibody
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纯化方法
- Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-AKT 1/2/3 (AA 401-479) antibody
适用: 人, 小鼠, 大鼠, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer473) antibody适用: 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 鱼 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer472) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 antibody适用: 人, 小鼠, 大鼠, Chimpanzee WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 14E5-16C8-25F6 unconjugated
anti-AKT 1/2/3 (pTyr474) antibody适用: 人, 小鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (AA 278-312) antibody适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB43841 unconjugated
anti-AKT 1/2/3 (AA 431-480) antibody适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: AKT Antibody is tested in western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Expect bands at ~56 kDa in size corresponding to AKT by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: User Optimized
IF Microscopy Dilution: 1:500 - 1:2,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Mouse model of metformin-induced diarrhea." in: BMJ open diabetes research & care, Vol. 8, Issue 1, (2020) (PubMed).
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: "Mouse model of metformin-induced diarrhea." in: BMJ open diabetes research & care, Vol. 8, Issue 1, (2020) (PubMed).
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- AKT 1/2/3
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别名
- AKT1, AKT2, AKT3
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背景
- Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
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基因ID
- 10000
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NCBI登录号
- NP_001014431
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UniProt
- P31749
抗原
-
