YY1 抗体 (AA 249-414)
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Quick Overview for YY1 抗体 (AA 249-414) (ABIN5563972)
抗原
See all YY1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 249-414
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原理
- YY1 antibody (pAb)
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纯化方法
- Affinity Purified
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免疫原
- This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.
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亚型
- IgG
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应用备注
- ChIP-Seq: 10 µL per ChIP IP: 0.5 - 4 µL per IP WB*: 1:1,000 - 1:5,000 dilution IHC (FFPE): 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- YY1 (YY1 Transcription Factor (YY1))
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别名
- YY1
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背景
- YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).
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分子量
- 63 kDa
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NCBI登录号
- NP_003394
抗原
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