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YY1 抗体 (AA 249-414)

The 兔 多克隆 anti-YY1 antibody (ABIN5563972) specifically detects YY1 in WB, IHC, IP, ChIP-seq 和 CUT&Tag. The antibody is reactive with 人 samples.
产品编号 ABIN5563972
发货至: 中国
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Quick Overview for YY1 抗体 (AA 249-414) (ABIN5563972)

抗原

See all YY1 抗体
YY1 (YY1 Transcription Factor (YY1))

适用

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宿主

  • 89
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克隆类型

  • 67
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多克隆

标记

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This YY1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)
  • 抗原表位

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    AA 249-414

    原理

    YY1 antibody (pAb)

    纯化方法

    Affinity Purified

    免疫原

    This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.

    亚型

    IgG
  • 应用备注

    ChIP-Seq: 10 µL per ChIP IP: 0.5 - 4 µL per IP WB*: 1:1,000 - 1:5,000 dilution IHC (FFPE): 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    YY1 (YY1 Transcription Factor (YY1))

    别名

    YY1

    背景

    YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).

    分子量

    63 kDa

    NCBI登录号

    NP_003394
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