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YY1 抗体

Cited in 1 publication. The 小鸡 多克隆 anti-YY1 antibody (ABIN2443872) specifically detects YY1 in WB 和 IF. The antibody is reactive with 人, 小鼠, 大鼠, 绵羊 和 Pig samples.
产品编号 ABIN2443872
发货至: 中国
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Quick Overview for YY1 抗体 (ABIN2443872)

抗原

See all YY1 抗体
YY1 (YY1 Transcription Factor (YY1))

适用

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人, 小鼠, 大鼠, 绵羊, Pig

宿主

  • 87
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小鸡

克隆类型

  • 65
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多克隆

标记

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This YY1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 原理

    YY1 Chicken Polyclonal Antibody

    特异性

    The antibody recognizes the Transcription Factor YY1, in WB, the antibody recognizes the O-glycosylated, the phosphorylated, and the post-translationally unmodified protein in cellular and tissue lysates.

    纯化方法

    Purified

    免疫原

    Recombinant human YY1
  • 应用备注

    WB 1:3000,IF 1:500

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    If reconstituted with deionized water in 30 μL: WB 1:3000 - 1:8000, IF 1:500 - 1:1000 (in tissue paraffin sections). Optimal dilution has to be determined by the user.

    缓冲液

    Lyophilized purified total IgY in PBS pH 7.4

    储存液

    Sodium azide, Streptomycin

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

    有效期

    24 months
  • Gelev, Zabolotny, Lange, Hiromura, Yoo, Orlando, Kushnir, Horikoshi, Paquet, Bachvarov, Schaffer, Usheva: "A new paradigm for transcription factor TFIIB functionality." in: Scientific reports, Vol. 4, pp. 3664, (2015) (PubMed).

  • 抗原

    YY1 (YY1 Transcription Factor (YY1))

    别名

    YY1

    背景

    YY1 is a ubiquitous and multifunctional zinc-finger transcription factor involved in the activation or repression of a large number of genes that are critical for basic biological processes of cell growth, development, differentiation, cell cycle and even programmed cell death (apoptosis). YY1 is an essential protein, deletion of YY1 leads to lethality in mouse embryos and disruption of one allele causing developmental defects.

    UniProt

    P25490
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