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AKT1 抗体 (pSer473)

Name: AKT1 抗体 (pSer473)
SKU: ABIN5557411
Availability: OutOfStock

This anti-AKT1 antibody is a 兔单克隆 antibody detecting AKT1 in WB, IHC (p), FACS 和 IF (cc). Suitable for 人, 小鼠和大鼠.

Lane 1: NIH/3T3 treated with PDGF, Lane 2: NIH/3T3 untreatedlysates probed with Akt1(Ser473) (12A1) Monoclonal Antibody at 1:1000 overnight at 4˚C. (AKT1 抗体 (pSer473))

3T3 cells were stained with Akt1(Ser473) (12A1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (AKT1 抗体 (pSer473))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded mouse lung section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (AKT1 抗体 (pSer473))

IHC (p)

3 images

产品编号 ABIN5557411

发货至: 中国

Quick Overview for AKT1 抗体 (pSer473) (ABIN5557411)

抗原

See all AKT1 抗体

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

单克隆

标记

This AKT1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

克隆位点

12A1

Discover our top product AKT1 Primary Antibody

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pSer473

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473:QF(p-S)YS.

亚型

IgG
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 122-443) antibody
AKT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E11 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
AKT1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibody
AKT1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
AKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1F7E10 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 适用: 人, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7G2 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
AKT1 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 4H12 unconjugated Recombinant Antibody

应用备注

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

别名

Akt1

背景

Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG
Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

基因ID

207

UniProt

P31749

途径

PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect