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EIF2AK2 抗体 (pThr446)

Name: EIF2AK2 抗体 (pThr446)
SKU: ABIN5557332
Availability: OutOfStock

This anti-EIF2AK2 antibody is a 兔单克隆 antibody detecting EIF2AK2 in WB, IHC (p) 和 FACS. Suitable for 人, 小鼠和大鼠.

Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates, Lane 2: Untreated Hela whole cell lysates, probed with PKR(T446) (10A1) Monoclonal Antibody at 1:1000 overnight at 4˚C. (EIF2AK2 抗体 (pThr446))

1 image

产品编号 ABIN5557332

发货至: 中国

Quick Overview for EIF2AK2 抗体 (pThr446) (ABIN5557332)

抗原

See all EIF2AK2 抗体

EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

单克隆

标记

This EIF2AK2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

克隆位点

10A1

Discover our top product EIF2AK2 Primary Antibody

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pThr446

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

Synthetic peptide derived from human PKR(T446), around 450-500aa (phospho T446).

亚型

IgG
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibody
EIF2AK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibody
EIF2AK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 329-551) antibody
EIF2AK2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6H3A10 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 224-502) antibody
EIF2AK2 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibody
EIF2AK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 11-42) antibody
EIF2AK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 519-550) antibody
EIF2AK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr446) antibody
EIF2AK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibody
EIF2AK2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr446), (pThr451) antibody
EIF2AK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

别名

PKR

背景

Synonyms: Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase RNA-activated, PKR, Tyrosine-protein kinase EIF2AK2, p68 kinase, EIF2AK2, PRKR
Background: IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2).

基因ID

5610

UniProt

P19525

途径

DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C