DDX58 抗体 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58)

Details for Product anti-DDX58 Antibody No. ABIN5555173
抗原
适用
人, 小鼠, 大鼠
147
65
30
2
2
宿主

119
27
2
2
克隆类型
多克隆
标记
This DDX58 antibody is un-conjugated
11
5
5
3
3
3
3
3
3
2
2
2
2
2
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
111
45
39
27
20
13
12
5
4
3
3
1
1
选项
免疫原 RIG-1 antibody was raised against human GST-tagged RIG-1 protein.
亚型 IgG
特异性 This antibody detects DEAD box protein 58.
交叉反应 (详细) Species reactivity (tested):Human, mouse, rat
纯化方法 Peptide affinity chromatography
抗原
别名 DDX58 (DDX58 Antibody 摘要)
背景 The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.Synonyms: DEAD box protein 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, Probable ATP-dependent RNA helicase DDX58, RIG-1, RIG-I, Retinoic acid-inducible gene 1 protein
基因ID 23586
UniProt O95786
途径 Activation of Innate immune Response, Hepatitis C
应用备注 ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
缓冲液 PBS containing 0.02 % sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
有引用在: Korolowicz, Iyer, Czerwinski, Suresh, Yang, Padmanabhan, Sheri, Pandey, Skell, Marquis, Kallakury, Tucker, Menne: "Antiviral Efficacy and Host Innate Immunity Associated with SB 9200 Treatment in the Woodchuck Model of Chronic Hepatitis B." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161313, 2017 (PubMed).

Gan, Hao, Idell, Tang: "Transcription Factor Runx3 Is Induced by Influenza A Virus and Double-Strand RNA and Mediates Airway Epithelial Cell Apoptosis." in: Scientific reports, Vol. 5, pp. 17916, 2016 (PubMed).