Topoisomerase II alpha 抗体 (C-Term)
Quick Overview for Topoisomerase II alpha 抗体 (C-Term) (ABIN5542013)
抗原
See all Topoisomerase II alpha (TOP2A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
特异性
- This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.
-
交叉反应 （详细）
- Does not rect with Mouse (WR19L, NIH/3T3), Rat (PC12, Rat-1) or Hamster (BHK) on Western blot.
-
纯化方法
- Protein A agarose
-
免疫原
- Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa
-
亚型
- IgG2a
-
-
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) antibody
TOP2A 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1435-1531) antibodyTOP2A 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E2 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1352-1493) antibodyTOP2A 适用: 人, 小鼠 WB, IHC, IF, StM 宿主: 小鼠 Monoclonal TOP2A-1361 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1100-1530) antibodyTOP2A 适用: 人, 大鼠 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6C12G12 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1100-1530) antibodyTOP2A 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6D4B2 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (Internal Region) antibodyTOP2A 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (N-Term) antibodyTOP2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (C-Term) antibodyTOP2A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1167-1196), (C-Term) antibodyTOP2A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB24346 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (Thr1343) antibodyTOP2A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Western blot: 0.1-0.5 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200-300 μg/mL of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary Microwave oven 2 times for 10 minutes aech in 10 mM citrate buffer ( pH 6.5). Immunocytochemistry: 1-10 μg/mL. Flow cytometry: 0.1-1 μg/mL (final contentration).
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
-
储存液
- Azide free
-
储存条件
- -20 °C
-
储存方法
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
-
-
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
-
别名
- top2a
-
背景
- < div dir=ltr data-font-name=g_font_p0_4 data-canvas-width=519.3466821444035> Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well < span style=font-size: 12px> as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.
-
UniProt
- P11388
-
途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
-
