FADD 抗体 (AA 1-208)
Quick Overview for FADD 抗体 (AA 1-208) (ABIN5541517)
抗原
See all FADD 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-208
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特异性
- This antibody reacts with human FADD. Occasionally doublet bands can be seen.
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交叉反应 （详细）
- Does not react with Mouse (NIH/3T3, WR19L, BaF/3, P19), and Rat (PC12).
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纯化方法
- Protein A agarose
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免疫原
- Recombinant full-length human FADD (1-208 a.a.)
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亚型
- IgG1
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anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 12-206) antibody
FADD 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 1-208) antibodyFADD 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 20-150) antibodyFADD 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5A6A2 unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 7-178) antibodyFADD 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 1-208) antibodyFADD 适用: 人 WB, IHC, ELISA, IF, ICC 宿主: 小鼠 Monoclonal J1D2 unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 1-208) antibodyFADD 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 109-208) antibodyFADD 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibodyFADD 适用: 小鼠 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (AA 1-80) antibodyFADD 适用: 人, 小鼠, 大鼠, 猴, Pig WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (pSer191) antibodyFADD 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot : 1 μg/mL for chemiluminescence detection system. For details see protocol below.
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实验流程
- SDS-PAGE & Western blotting 1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50 mM Tris-HCl, pH 7.2, 250 mM NaCl, 0.1 % NP-40, 2 mM EDTA, 10 % glycerol) containing appropriate protease inhibitors. Incubate it at 4 o C with rotating for 30 minutes, then sonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4 o C and transfer the supernatant to another tube. Measure the protein concentration of the supernatant and add the Lysis buffer to make an 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli's sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μ L of the sample per lane in a 1 mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20 % MeOH). See the manufacture's manual for specific transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature, or overnight at 4 o C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7.2 containing 1 % skimmed milk as suggested in the APPLICATIONS for 1 hour at room temperature. (The optimal antibody concentration will depend on the experimental conditions.) 8) Wash the membrane with PBS-T [0.05 % Tween-20 in PBS] (5 minutes x 6 times). 9) Incubate the membrane with the 1:10,000 HRP-conjugated anti-mouse IgG diluted with 1 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriate chemilumi nescence reagents for 1 minute. Remove extra reagent from the membrane by dabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 1 minute. Develop the film as usual. The conditions for exposure and development may vary. (Positive controls for Western blotting HeLa, MRC-5, ZR-75-1, A431, HEp-2)
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS ( pH 7.2) / 1 % Sucrose. Contains no preservatives.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Prior to reconstitution store at 2-8°C. Following reconstitution store at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
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别名
- fadd
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背景
- FADD (Fas-associated death domain protein)/MORT1 has been identified as a protein that associates specifically with the intracellular domain of Fas. It contains both a death doma in (DD) and a death effecter domain (DED). DD is found in several death-inducing receptors of the TNF family, including Fas (CD95/APO-1) and TNFR-1. DED is found at the N-terminus of FADD and it is also present within the ICE-like protease, caspase-8 (FLICE/Mch5/MACH) and caspase-10 (FLICE2). Both DD and DED are able to associate with homologous regions in other proteins, and thus prompt binding of such proteins to one another. Upon activation, Fas relays death signals through DD, which interacts with the DD of the adaptor molecules FADD, recruiting them to the membrane. FADD then associates with caspase-8 through DED, leading to the assembly of a death-inducing signaling complex (DISC). DISC-associated caspase-8 subsequently initiates proteolytic activation of other caspase, which in turn leads to apoptosis.
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UniProt
- Q13158
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途径
- Apoptosis, TLR signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
抗原
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