Sonic Hedgehog 抗体 (N-Term)
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Quick Overview for Sonic Hedgehog 抗体 (N-Term) (ABIN5540856)
抗原
See all Sonic Hedgehog (SHH) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- This antibody detects Mouse Shh with Western Blot. Species: Mouse.
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纯化方法
- Affinity Chromatography on Protein A/G
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免疫原
- Recombinant Mouse Sonic Hedgehog (Shh) N-Terminal fragment.
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亚型
- IgG2
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anti-Sonic Hedgehog (SHH) (AA 21-120) antibody
SHH 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 26-161) antibodySHH 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H4 unconjugated
anti-Sonic Hedgehog (SHH) (C-Term) antibodyVerified SHH 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 豚鼠, 马, 山羊, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 36-193) antibodySHH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 36-193) antibodySHH 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Sonic Hedgehog (SHH) (AA 58-91), (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB52387 unconjugated
anti-Sonic Hedgehog (SHH) antibodySHH 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 24-197) antibodySHH 适用: 人, 大鼠, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 10H6 unconjugated
anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: 1/250-1/1000. Neutralization. Immunohistochemistry on Frozen Sections: 1/50-1/200.
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限制
- 仅限研究用
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缓冲液
- Purification: Affinity Chromatography on Protein A/G
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- Sonic Hedgehog (SHH)
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背景
- Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage. Although the binding affinity to their receptors is not changed, lipid-modified Shh-N proteins are more potent than the unmodified proteins in cell-based assays. Other hydrophobic modifications to unmodified Shh-N, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase Shh-N potency.
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UniProt
- Q62226
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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