Actin 抗体 (N-Term)
Quick Overview for Actin 抗体 (N-Term) (ABIN5540760)
抗原
See all Actin (ACTA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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序列
- Ac-DEDETTALVC -COOH
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特异性
- This 3B3 Monoclonal antibody is highly specific for Alpha-Skeletal Actin, and does not cross react with other Actin isoforms.
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纯化方法
- Purified
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免疫原
- Peptide comprising the N-terminal nonapeptide of alpha-skeletal Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.
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亚型
- IgG1
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应用备注
- ELISA. Flow Cytometry. Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Buffer System: PBS Preservatives: 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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有效期
- 12 months
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- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
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别名
- alpha skeletal muscle actin,acta1
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背景
- Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.
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途径
- Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
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