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Actin 抗体 (N-Term)

This 小鼠 单克隆 anti-Actin antibody (Clone 3B3) specifically detects Actin in WB, IHC (p), IHC (fro) 和 EIA. The antibody is reactive with 人, 大鼠, 兔 和 Pig samples.
产品编号 ABIN5540760
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Actin 抗体 (N-Term) (ABIN5540760)

抗原

See all Actin (ACTA1) 抗体
Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

适用

  • 105
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  • 1
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人, 大鼠, 兔, Pig

宿主

  • 92
  • 31
小鼠

克隆类型

  • 74
  • 46
  • 1
单克隆

标记

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  • 12
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This Actin antibody is un-conjugated

应用范围

  • 98
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  • 21
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  • 1
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

3B3
  • 抗原表位

    • 12
    • 8
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    • 5
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    • 1
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    N-Term

    序列

    Ac-DEDETTALVC -COOH

    特异性

    This 3B3 Monoclonal antibody is highly specific for Alpha-Skeletal Actin, and does not cross react with other Actin isoforms.

    纯化方法

    Purified

    免疫原

    Peptide comprising the N-terminal nonapeptide of alpha-skeletal Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.

    亚型

    IgG1
  • 应用备注

    ELISA. Flow Cytometry. Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer System: PBS Preservatives: 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

    别名

    alpha skeletal muscle actin,acta1

    背景

    Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.

    途径

    Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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