Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
纯化方法
Affinity purification using a column to which the native protein was coupled
SYN1
适用: 人, 大鼠
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
Western blot: 1/1000. Immunohistochemistry / immunoflourescence: 1:1000. Immunoprecipitation: 2 μL per 200 μg lysate.
限制
仅限研究用
状态
Liquid
缓冲液
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol
储存条件
4 °C,-20 °C
储存方法
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
SYN1
(Synapsin I (SYN1))
别名
synapsin-1
背景
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002 Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989 Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001 Kao et al., 2002).