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EPAS1 抗体 (N-Term)

Name: EPAS1 抗体 (N-Term)
SKU: ABIN5540327
Availability: OutOfStock

This anti-EPAS1 antibody is a 兔多克隆 antibody detecting EPAS1 in WB, ICC, IF, IHC (p) 和 IHC (fro). Suitable for 大鼠和小鼠.

产品编号 ABIN5540327

发货至: 中国

Quick Overview for EPAS1 抗体 (N-Term) (ABIN5540327)

抗原

See all EPAS1 抗体

EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

适用

大鼠, 小鼠

宿主

兔

克隆类型

多克隆

标记

This EPAS1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product EPAS1 Primary Antibody

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AA 202-240, N-Term

序列

YNNCPPHSSL CGYKEPLLSC LIIMCEPIQH PSHMDIPLD

特异性

This antibody detects HIF2A / HIF2 alpha (202-240). No cross reactivity with other proteins.

纯化方法

Immunogen Affinity Chromatography

免疫原

A synthetic peptide corresponding to a sequence at N-terminus of Rat HIF-2-alpha (202-240)

亚型

IgG
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 339-541) antibody
EPAS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 21-336) antibody
EPAS1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (Ser343) antibody
EPAS1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 680-870) antibody
EPAS1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1H3E12 unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (C-Term) antibody
EPAS1 适用: 人, 大鼠, 小鼠, 鱼 WB, IHC, ELISA, IP, ICC, FACS, IF, ChIP, GS 宿主: 兔 Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 28-347) antibody
EPAS1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G2 unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 588-870) antibody
EPAS1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) antibody
EPAS1 适用: 人 WB, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody
EPAS1 适用: 人, 大鼠, 小鼠, Hamster WB, IHC, ELISA, IP, ICC, FACS, IF, ChIP 宿主: 小鼠 Monoclonal ep190b unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 1-130) antibody
EPAS1 适用: 人, 大鼠, 小鼠, 猴, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot: 0.1-0.5 μg/mL with the appropriate system to detect HIF-2α in cells and tissues. Immunohistochemistry on Paraffin Sections: 0.5-1 μg/mL to detect HIF-2α in formalin fixed and paraffin embedded tissues. Bioling the sections is required. Immunohistochemistry on Frozen Sections: 0.5-1 μg/mL to detect HIF-2α in formalin or acetone fixed tissues.

限制

仅限研究用
缓冲液

5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg Sidium Azide

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

有效期

12 months
抗原

EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

别名

hif2a,hif2 alpha

背景

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration.

途径

Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect